5-[3-(Aarboxymethoxy)Phenyl]-3-(4,5-Dimethyl-2-Thiazolyl)-2-(4-Sulfophenyl)-2H-Tetrazolium Inner Salt(CAS 138169-43-4)

The primary application of 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium is in cell viability and proliferation assays. It is a key component of the CellTiter 96® AQueous One Solution Cell Proliferation Assay, which is used to determine the number of viable cells in proliferation, cytotoxicity, or chemosensitivity assays.

This compound offers several advantages in cell-based assays:

• It produces a water-soluble formazan, eliminating the need for a solubilization step.
• It can be used in a "one-step" assay format, simplifying the procedure.
• It allows for high-throughput screening due to its simple "add-incubate-measure" format.
• It provides flexibility, as assay plates can be read and returned to an incubator for further color development.

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