structure of 1609-47-8

Diethyl pyrocarbonate

CAS No.: 1609-47-8 Brand: MolBest
M. Ft.: C6H10O5 Cat. No: G3884563
M. Wt.: 162.14 MDL: MFCD00009106
Storage: 2-8°C
Purity Grade Package Stock Price Quantity
99% RG 5G In Stock 4 USD
99% RG 100G In Stock 27 USD
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Applications of 1609-47-8

Diethyl pyrocarbonate finds extensive applications in biochemistry and molecular biology:

  • Inactivation of Ribonucleases: It is primarily used to prepare ribonuclease-free water and buffers essential for RNA work.
  • Protein Modification: Researchers use it to study the role of specific amino acids in enzymes by selectively modifying histidine residues.
  • Nucleic Acid Research: It serves as a tool for probing the structure of nucleic acids, allowing scientists to investigate the effects of modifications on RNA and DNA stability and function.

Biological Activity of 1609-47-8

The primary biological activity of diethyl pyrocarbonate lies in its role as a non-specific inhibitor of ribonuclease enzymes. By modifying critical amino acids in these enzymes, diethyl pyrocarbonate effectively prevents them from degrading ribonucleic acid molecules. This property is particularly valuable in laboratory settings where maintaining RNA integrity is essential for downstream applications such as reverse transcription polymerase chain reaction and RNA sequencing.

Moreover, diethyl pyrocarbonate has been shown to influence enzyme activity and protein conformation. For example, it can alter the enzymatic activity of certain proteins by modifying histidine residues, which are often crucial for catalytic function.

NMR spectrum of 1609-47-8

Physical sample testing spectrum (NMR) of 1609-47-8

Safety Information of 1609-47-8

Pictograms

Signal Word

Warning

Hazard Statements

H302 Harmful if swallowed
H315 Causes skin irritation
H319 Causes serious eye irritation
H335 May cause respiratory irritation

Safety Data Sheet

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Bulk Packaging Price Explanation of 1609-47-8

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Purchase Instructions of 1609-47-8

All products are for research and development use only, not for any other uses, and must be handled by technically-qualified persons.

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