2,5-Dioxopyrrolidin-1-Yl 3-(4-Hydroxyphenyl)Propanoate

Bolton-Hunter reagent is primarily used in:

• Protein iodination: Labeling proteins for detection and quantification.
• Binding assays: Studying protein interactions and affinities.
• Immunological studies: Facilitating the development of radioimmunoassays.
• Research involving non-tyrosine containing proteins: Expanding the scope of protein studies beyond traditional methods.

The water-soluble variant, known as sulfo-Bolton-Hunter reagent, enhances solubility and reactivity, making it suitable for a broader range of biological applications.

Bolton-Hunter reagent exhibits significant biological activity primarily through its role in protein labeling. By enabling the iodination of proteins that do not contain tyrosine residues, it expands the range of proteins that can be studied using radioactive labeling techniques. This capability is particularly useful in studying protein interactions, localization, and dynamics within biological systems. The resultant iodinated proteins can be traced and quantified in various experimental setups, making them valuable tools in biochemical research.

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