2-Ethyl-5-Phenylisoxazolium-3-Sulfonate

Woodward's Reagent K is widely used in:

• Peptide Synthesis: It serves as a coupling reagent that enhances the efficiency of forming peptide bonds.
• Biochemical Assays: Used for modifying proteins and studying enzyme kinetics.
• Chemical Biology: Employed in research involving nucleophilic reactions and protein modifications.

Woodward's Reagent K has been shown to interact with various biological molecules. Notably, it can inactivate enzymes such as L-threonine dehydrogenase in Escherichia coli, leading to significant changes in enzymatic activity and absorbance properties. This property suggests potential applications in studying enzyme mechanisms and metabolic pathways.

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